RNA Methyltransferases in Antibiotic Resistance.The transfer of well-studied native and chimeric pattern recognition receptors (PRRs) to susceptible plants is a proven strategy to improve host resistance. Cuartas, Diana Carolina Castaño, Mauricio Corredor. Crystal structure of ErmE - 23S rRNA methyltransferase in macrolide resistance. rRNA Methylation and Antibiotic Resistance. Journal of Biological Chemistry 2020, 295 Shared requirements for key residues in the antibiotic resistance enzymes ErmC and ErmE suggest a common mode of RNA recognition. Photoelectrochemical water splitting coupled with degradation of organic pollutants enhanced by surface and interface engineering of BiVO4 photoanode.
Jingchao Liu, Jianming Li, Yanfei Li, Jian Guo, Si-Min Xu, Ruikang Zhang, Mingfei Shao. The conserved aspartate in motif III of β family AdoMet-dependent DNA methyltransferase is important for methylation. Aathira Gopinath, Manasi Kulkarni, Ishtiyaq Ahmed, Om Prakash Chouhan, Kayarat Saikrishnan. Potential Target Site for Inhibitors in MLSB Antibiotic Resistance. Hak Jin Lee, Seong Tae Jhang, Hyung Jong Jin. Organic & Biomolecular Chemistry 2021, 19 Deciphering protein microenvironment by using a cysteine specific switch-ON fluorescent probe. Jessy Mariam, Anila Hoskere Ashoka, Vandana Gaded, Firoj Ali, Harshada Malvi, Amitava Das, Ruchi Anand. Structural basis of successive adenosine modifications by the conserved ribosomal methyltransferase KsgA. Niklas C Stephan, Anne B Ries, Daniel Boehringer, Nenad Ban. Three critical regions of the erythromycin resistance methyltransferase, ErmE, are required for function supporting a model for the interaction of Erm family enzymes with substrate rRNA. Journal of Biological Chemistry 2022, 298 Crystal structure and functional analysis of mycobacterial erythromycin resistance methyltransferase Erm38 reveals its RNA-binding site. Boon Chong Goh, Xinyu Xiang, Julien Lescar, Peter C. Decoding the Mechanism of Specific RNA Targeting by Ribosomal Methyltransferases. This article is cited by 12 publications. This work explores the factors that govern the emergence of resistance and paves the way for the design of specific inhibitors useful in reversing antibiotic resistance. Moreover, in vivo studies confirmed that chimeric constructs are competent in imparting macrolide resistance. 
The results revealed that specific loop embellishments on the basic Rossmann fold are key determinants in the selection of the cognate RNA. With a combination of evolutionary and structure-guided approaches, a set of chimeras were created that altered the targeting specificity of KsgA such that it acted similarly to erythromycin-resistant methyltransferases (Erms), rMtases found in multidrug-resistant pathogens. In this work, KsgA, a housekeeping ribosomal methyltransferase (rMtase) involved in ribosome biogenesis, was exploited as a model system to delineate the specific targeting determinants that impart substrate specificity to rMtases. Post-translational methylation of rRNA at select positions is a prevalent resistance mechanism adopted by pathogens.
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